Dr. Felicia B. Axelrod’s Report
DYSAUTONOMIA DAY—June 2001

Familial Dysautonomia: New Hope For Our Tomorrows!

This year we celebrated our 16th International Dysautonomia Day and it marked a very special one. For this year we received the news we have all wanted to hear. The FD gene has been found and we are truly indebted to the researchers who have persisted in this endeavor!

We have come a long way in the past 10 years. 1990 was an extremely important year. It was the official start of the Human Genome Project when scientists all over the world collaborated for the common goal of eventually sequencing the entire human genome. It was also the year that the Dysautonomia Foundation enlisted the assistance of Dr. Gusella’s laboratory. (Dr. Gusella’s laboratory was already a part of the Human Genome Project and he agreed to dedicate part of his laboratory to finding the FD gene). Then, in the year 2000, the human genome was sequenced and in January 2001 the FD gene was identified.

With the identification of the FD gene, general population screening is a reality but more importantly we can start working toward development of new and better treatments for individuals affected with FD.

As Dr. Slaugenhaupt has indicated, IKBKAP is the name of the gene, IKAP is the protein product and two mutations can cause FD. In addition, Dr. Slaugenhaupt has demonstrated that there is tissue-specific expression in FD. The FD patient seems to be able to make some normal as well as abnormal IKAP in many tissues of the body but only abnormal IKAP in neural (brain) tissue. The most common mutation (also called the major mutation) does not seem to cause marked changes in the amount of normal IKAP made in skin or blood cells but practically eliminates normal IKAP in the brain. Instead an abnormal IKAP is made which probably is not able to function to full capacity.

What is the result of a poorly functioning IKAP? This is the major question. At present it is suspected that IKAP may work with other proteins and help other genes function. This would cause multiple other problems and explain the marked variability in expression of FD.

At this point basic research and clinical research will have to work together to understand the FD gene’s function so that we can understand the following and treat the affected FD patient:

  • Generally—How does IKAP and its coworker proteins affect development and maintenance of sensory and autonomic nervous systems?

  • Specifically—How does IKAP and its coworker proteins cause FD?

  • Can the FD patient start to make IKAP (or other affected genes and proteins) in neural tissue?

Basic research will probably include a number of different approaches. In addition to the development of animal models, such as the mouse and fruit fly, there will be laboratory experiments to understand the chemistry of cellular action and interactions. It will also be important to continue pathological examinations in order to correlate the new genetic findings with expression in patient tissues.

Most of the pathological advances occurred from 1969 to 1980. In this period, major contributions by Dr. John Pearson suggested that FD was a neurodevelopmental disorder—a disorder resulting from incomplete development of a special portion of the nervous system. The affected nerve cells were the unmyelinated and small myelinated neurons which eventually provide sensory function (pain and temperature perception) and autonomic function (sympathetics to help cope with stress). However, all of the reported findings were in the peripheral nervous system and obviously this is not the complete story, since we know that in the brain there are many autonomic centers. In addition, the clinical manifestations and the genetic findings indicate that the central nervous system (the brain) is also involved in FD. The question then is whether the decreased nerve cells in the peripheral autonomic nervous system are part of the original problem in development, or if they are a secondary loss because the nurturing central neurons are decreased.

To help understand more about nerve supply, i.e. which specific types are affected, this spring Dr. Hilz and I did a study with Dr. Kennedy from the University of Minnesota. Skin biopsies were taken from two sites (the back and calf) from 10 FD patients. The results were as follows:

Epidermal nerve fibers were markedly decreased in back and calf

Dermal nerve fibers to subepidermal neural plexis were markedly decreased

Sweat glands were decreased and those present had decreased innervation

In the remaining subepidermal nerve fibers there was a complete absence of Substance P and Calcitonin gene-related peptide. Normally, using special stains, 10% of subepidermal nerve fibers will contain these proteins. These proteins help transmit pain sensation

However, there was positive staining for vasoactive intestinal peptide in 9 of 10 skin biopsies from the back. This protein is not normally seen and only is seen when there is reinervation (or new nerve growth).

Clinical research is ongoing. One of the goals of clinical research at the Dysautonomia Treatment and Evaluation Center is to correlate clinical symptoms and genetic findings through studies. In general, clinical studies are important because they help buy time—they provide a means of helping patients until definitive therapies are found.

One type of study is prospective and requires ongoing evaluation of effect. The second type is to analyze data in retrospect. The prospective type can be used to help us understand the gene and the wide variability of expression or for assessment of drug effect—either a new drug or a new use of an old drug. The retrospective clinical study helps to evaluate interventions and determine efficacy and impact.

For the past 30 years, the Dysautonomia Center has appreciated that although the FD patient has many different problems, there are four body systems that continue to be especially problematic—gastrointestinal, respiratory, cardiovascular and neurological. Of these four the gastrointestinal problems are especially distressing and difficult to manage, especially the dysautonomia vomiting crisis.

Prospective studies: This has included assessment of autonomic and sensory function in our patients and Dr. Hilz has been very helpful in this area. We have studied the FD patients’ response to breathing low oxygen and high carbon dioxide, variability in pain and temperature sensations, and blood flow in the brain under various provocations such as change in position. This will be extremely important in eventually assessing impact of future therapies. Other prospective studies have been directed to trying new drug therapies. Some drugs were shown not to be helpful but some have been helpful. Midodrine (proamatine) is one example of a study drug that was found to be helpful in treatment of low blood pressure.

Over the past few years advances have also been made in our treatment of the dysautonomia vomiting crisis. It is now appreciated that the cause of vomiting (and nausea) is not primarily due to a problem with the gastrointestinal system (the stomach or the intestines)—rather vomiting and nausea appear to be caused by the brain’s excessive reaction to stress (due to infection, visceral pain, anxiety or even the stress of arousal from sleep). In some ways the brain is acting like it is having uncontrolled discharges—similar to a type of seizure. In this case instead of arms or legs reacting, the individual’s internal organs react. This is like an autonomic seizure with nausea being the “aura.”

Prevention of the crisis relies on understanding the particular stress and either avoiding that stress or treating it early. However, once the crisis has begun, Valium is still the most effective drug and one that neurologists frequently use in emergency situations to control seizures. Other medications that modify brain activity have also been helpful—drugs that modify response to stress (such as Catapres) and, in some selected cases, more classical anticonvulsants (seizure medications) have been helpful.

Retrospective studies: These studies are an excellent means of evaluating past interventions but they require three essential factors—a critical number of participants, a sufficient amount of time, and a dedicated statistician. As of January 2001, the Center had over 550 registered patients, had thirty years of data, and had started to work with a staff of statisticians led by Dr. Judith Goldberg. The areas presently being evaluated are growth hormone therapy, medications and treatments and survival and quality of life. The preliminary results are as follows:

Growth Hormone Therapy: Assessment in this area is extremely problematic since only 22 patients have been treated and there were 20 different physicians involved. Testing, medication, and doses vary widely. However, we are trying to obtain and interpret the data. We also understand that we will need new growth and weight charts for all our patients because there is a coexisting impact from our ability to provide better nutrition through gastrostomy feedings, as well as treatment of spine curvature.

Medications and Treatment: The medications that we plan to review are the ones that are used widely to treat blood pressure problems—Florinef, proamatine (midodrine), and Catapres (clonidine). The surgical interventions that we plan to review are fundoplication, tear duct cautery and tonsillectomy and adenoidectomy.

Survival and Quality of Life: Some preliminary results from these data are already available. The first review of survival was in 1965. This study indicated that 50% of children born with FD did not survive past 5 years of life. However, our own review in 1982 indicated that even supportive treatment was making an impact because 50% of patients could expect to survive to at least age 30 years.

As of January 2001, the Center’s registry had more than doubled from the 1982 database and the Israeli population had been added, so it was appropriate to review our results again. Of the 551 patients, 273 were males and 277 were females. Although 30.5% of patients were from the Greater NYC area (New York, New Jersey and Connecticut), patients from other areas were included:

                          United States and Canada     63.5%
                         
Israel                                   30.5%
                         
Other                                   6.2%

The data from prior to March ’81 were considered the “old data” and were compared to the data obtained from March ’81 to January ’01, the “new data.” The “new data” were somewhat better as overall survival improved. The 50% mark had been shifted to age 40 years. We also learned that neither sex or geographic location appeared to impact on survival. What was especially significant was the length of time that a patient was followed by the Center. For example, in the “old data,” if a patient was followed for 10 years, one could expect 65% survival, whereas in the “new data” survival

increased to 80%. Therefore patients who entered the Center after March ’81 are receiving benefits that markedly improve their prognosis.

We are not sure what has caused the improvement. Among the possibilities being considered are increased expertise of Center personnel, use of particular treatments (Valium use started in 1970 but Florinef started in 1985), fundoplication and gastrostomy (started in 1980), and establishment of the Israeli Center (1982). It may be one or a combination of the above.

Quality of life factors are still being assessed. At the present time 24 patients have married, there have been a total of 15 children born to individuals with FD (all children are healthy), and 37 patients drive. We are still analyzing educational levels, independent living and types of employment.

The finding of the FD gene has certainly brought us closer to more definitive treatments—either directly by gene or protein or enzyme replacement or indirectly through medications that can induce function. In the interim we are making progress in our supportive treatments. However, we will have to continue to evaluate present treatments, continue proven beneficial programs, and continue research and studies.

Finally, we cannot think about the future of FD without appreciating the fact that we have a wonderful expanding number of adult patients. This is one of the best testaments to the effectiveness of our advances in treatment. Not only does their number increase each year but also their personal accomplishments become more impressive.

Among my personal joys I must admit the FD adult population accounts for a large proportion. Although I strongly believe that all of the FD adults are very special, since 1993 the Dysautonomia Center has been selecting a few of these special individuals for special mention.

None of us knows what the future has in store or what challenges we might have to face in the future. However, what we can do is look to others who have shown us by example that there are many facets to an individual and that there are various ways to make the most of one’s own potential. The individuals that I have chosen in the past, as well as the ones that have been chosen today, have accepted FD and then gone beyond it—they have shown great courage and have taken pride in their own accomplishments. With that inner strength, they have developed independence and self esteem. They are an inspiration to other individuals with FD because they are the leaders and the pioneers and are showing the way. To date 20 individuals have been so recognized.

                    Rachel Berger                       Jason Gross
                    Burt Levy                              Roxanne Stein
                    Ittai Bergman                        Leigh Gurewitz
                    Robert Newman                    Amy Trinkler
                    Dede Coplin-Lipsitz                Martin Jabenis
                    Kim Segal                             Naomi Walker
                    Judy Fettman                        Jacob Krieger
                    Debra Schoenholz                  Michael Weiss
                    Jackie Gage                          Alan Kudler
                    Brian Solomons                     Brett Zinman

This year I have added three more individuals. This year’s DYS-tinguished FD Adults are Veronica Segal, Andrew Sigman, and Lori Kaplan. All three of these individuals have worked hard to overcome some of their problems and have made a real effort to reach out and communicate with other FD individuals to encourage and help. They have accomplished their goals with a great deal of self-reliance and courage and they are excellent role models. I believe much of their success is due to their ability to accept FD and go beyond their anger at being affected. They concentrated instead on greater goals—work, jobs, school, friends and family. They are an inspiration and should be applauded.

* * * * *

Now on a personal note I would like to thank a few individuals. First of all my staff—Dena Berlin, Alice Chaikin and Philip Giarraffa. They are essential and make the Center run efficiently and with a level of expertise that is incredible. I would also like to thank Lenore Roseman and Maryon Weill who are the heart and soul of an organization that is unique. They give 150% of themselves on a daily basis and I am sure we would not have come so far without them. Finally I want to thank the Dysautonomia Foundation as a whole and in particular the Board and their visionary Presidents. Over the years some hard decisions and choices had to be made. But these presidents, who work tirelessly without financial compensation, and rarely, if ever, are thanked for their efforts, maintained their vision and their optimism and made all the correct choices. These choices have brought us to the threshold of a new era for FD—one that will challenge us but will bear incredible rewards for our FD population.

“Success is not a place at which one arrives, but rather…the spirit with which one undertakes and continues the journey.”

—Alex Noble, “In Touch with the Present”

Let us continue our journey with continued hope!